Tojo virus

Setiap masyarakat ekonomi menengah ke bawah yang memegang kartu tersebut, berhak mendapat pelayanan kesehatan secara gratis, utamanya berobat. Dia menerangkan 3. Ia mengakui cukup banyak warga kurang mampu di daerah tersebut namun menderita penyakit yang perawatan dan pengobatannya tidak ditanggung dalam mekanisme BPJS Kesehatan. Kirumi then explained what happened between her and Ryoma's meeting, about how he gave up his life to her, before the Monokuma Kubs showed everyone Ryoma's motive video, showing everyone how he had lost his will to live.

Despite being found guilty however, Kirumi secretly hoped that someone would take the fall for her. As a result, she continued to manipulate everyone, but Kokichi saw through her act and called her out in front of everyone. Kirumi was irritated by this and called him a detestable cretin. Kaito did not blame her however and merely stated that she was trying to live in her own way. After Monokuma announced that she will be executed shortly, she sprinted away from the trial room. She was desperately trying to live for both her nation and her people, and everyone cheered her on, telling her to escape.

However, all of their cheering was pointless. Instead of Monokuma dragging her into her execution , she ran straight into it, playing off of her desire to live and escape. Everyone was horrified by the way she was killed, but gradually came to the realization that the motive videos were far more dangerous than they had originally believed if they had the power to drive Kirumi to go that far.

As Makoto explained that she was a very talented person as a maid and athlete, Kirumi humbly denied his words, citing them as an overstatement. Later, Kirumi cleaned all the students' dormitories before the incident of Yasuhiro Hagakure 's murder. She stated that she didn't see the culprit during that time, and Tsumugi's testimony proved Kirumi's alibi. During the investigation, Kirumi guarded the crime scene along with Hajime Hinata.

Kirumi is a tall, thin young woman with pale, ashy, sage-colored hair and long bangs that obscure her left eye but her left eye is occasionally visible through it when she's expressing certain emotions.

Her skin is fairly pale, and her eyes are of a pale, warm green color. Her maid uniform consists of a long black apron dress in a pinafore style with a spiderweb motif possibly foreshadowing her execution , four white buttons along the middle of the torso of her dress, and the button of her previous high school's emblem on the left.

A white dress is donned underneath her apron dress with a black collar, and a purple tie around her neck.

The sleeves of her shirt have elegant frills at the end, which goes along with the bubble skirt appearance of her dress. She wears a black headdress with black lace and frills attached to it, with four white pins on it, a common appearance that maids take up. She wears black, form-fitting gloves with silver rings attached to the back of her hands. Underneath her dress is a chic-combination of a petticoat with black lace and black tights, which she deems practical by not exposing her legs.

In the Prologue, Kirumi wears a cadet blue sailor school uniform with a long pleated skirt, a red tie, red stripes on the collar, black tights with black inside shoes on top, and a brown headband that has a small bow on the right side of her hair. Kirumi is a refined, mature young lady who is described as "a professional who does a perfect job".

She has a keen mind and she is talented in various ways, reportedly able to carry out any job, as well as being capable of independence and doing things on her own. She is polite and formal, as well as quite serious, and very willing to use all of her power to cooperate with the other students. She is intelligent, observant, and level-headed. Her intelligence can be shown when she is speculating. Her speculations are provided with a fact and thus they are quite hard to refute—for example, when she accused Gonta of being the culprit of Rantaro's murder case everyone believed her until Kaede objected it with a solid evidence.

In Chapter 2, when the students discovered the half hallway of 2nd floor and the third floor, she felt that no one had set foot on the academy for a quite long time based on small details, and when she found out that the rooms of the deceased are locked by Monokuma, she thought that it was needed to be done in order to secure Rantaro's belongings, since Monokuma himself mentioned that he may have had a hunch about the truth behind the Killing Game.

As a maid, Kirumi's creed is "selfless devotion". Thus, she has a very strong sense of duty and a will to work for the sake of others even during the Killing Game. She voluntarily does house work such as cleaning and preparing meals for the students, even making sure each time that there's both Japanese and Western cuisine. Because of this, the other students seem to have strong trust in her, and she tends to wait outside the dormitory in case anyone has any requests for her.

She is also shown to be very considerate and often asks the others if they are doing all right and gives them some advice. Her concern and kindness is often described as "motherlike", with Kokichi and Gonta even asking her to be "their mom". However, being a high school student, Kirumi completely denies such comparisons. Even in her last Free Time Event with Kaede, she stated that she dislikes being called the "mom" of the group, and in one of her Free Time Event with Shuichi, she said that being called a mom can be taken as an insult for some women, including her.

On the other hand, Kirumi lacks the concept of doing something for herself, and for her even to have a conversation is a request. She believes her own opinions hold no importance. Despite her sense of duty, she does have a limit to what she is willing to do, declining requests such as destroying a nation, giving sexual services, or unfairly removing someone who hasn't done anything wrong from a public area.

She has stated that while she is a maid, she is not a slave. In her Love Suite Event , it's shown that she wishes to be treated as an equal by a kind master she loves, but she fears it will hinder him and she feels guilty for having such feelings.

When she and her feelings are fully accepted by him, she finally lets her calm composure break and cries like a child in relief. For the sake of the people who need her, Kirumi is willing to do some immoral acts like murder and manipulation if it is absolutely necessary in her mind.

She is willing to bear the burden of being seen as evil for the sake of her nation and its people. She claimed that she murdered Ryoma after he turned around, convinced that he already knew what was going to happen and had surrendered to his fate, but she possibly just used this way of thinking as a way to excuse her actions to herself and others. She has a great sense of determination, so much so that during her execution, she went out of her way to escape the entire situation rather than just succumbing to death, believing that she cannot die until she makes sure that her people are safe.

It's also heavily implied that she voted for Shuichi during the voting time merely out of spite because he was the one who exposed her and ruined her plan, showing that she does possess a petty side despite trying to appear dignified. Similarly, when her composure begun to break under correct accusations, she turned much more intense and aggressive, and used insulting words towards her opponents. In the end, her final moments were described as completely undignified, though Kokichi gave her some respect for attempting to run away in order to live.

Kirumi's talent as the Ultimate Maid is a fabrication made by Team Danganronpa. Due to that fact, it is unclear whether Kirumi is a multi-talented maid in the past. While working a part-time job as a maid, Kirumi is able to carry out any job, and has created a reputation for doing perfect work. In several occasions during the Killing Game, she could sense her classmate's thoughts and offered them personal services such as bringing food to Maki's room, suggesting invention ideas to Miu, and helping Korekiyo to record his observations.

She is also talented at various sports. She takes care of cooking and cleaning for the other students. She quickly adapted to the atmosphere of the Killing Game and stated that even though she can't perform surgeries, she has a medical experience and she can use the medical supplies in the warehouse.

In her Free Time Events , she mentioned that the last person she served before getting kidnapped to the Academy was an author of light novels who was able to publish a novel per month after taking Kirumi's advice on writing quickly.

Before him, Kirumi served a politician as a maid and his mahjong's mentor who managed to become the Prime Minister in a country with a peculiar system to decide everything using mahjong. One of the biggest case she had ever experienced was when she served an owner of a conglomerate who got his daughter was kidnapped by criminals.

Using his daughter, the criminal organization asked for a ransom equaling the state budget. Wishing to avoid giving money to the criminals, Kirumi set foot to the criminals' main base herself and was able to neutralize the enemy forces one by one. Eventually, Kirumi persuaded the criminal to start a business who later accepted her proposal and together with the Ultimate Maid he is now maintaining a butler industry. Being a very talented maid, Kirumi doesn't want to be called as a perfect maid machine as she claimed that she is also an inexperienced high schooler.

She also mentioned that there was a time where she was unable support people without potentials. She worked as a businessman's faithful maid and accompany him to his success, to the point he got the nickname of the "Hero of our Era". However, his dependency on Kirumi proved to be his weakness as he couldn't do anything by himself without her on his side.

Her principles of Selfless Devotion stems from this experience. Her other weakness is the fact that she can't cut konjac. Kirumi strongly believed in Kaede and was impressed with how well she was able to keep the whole group together as well as how she was able to keep a killing from happening during Monokuma's time limit motive.

After Kaede was revealed as Rantaro's killer, this strong sense of trust and belief did not waver. Kirumi stated that despite the fact that she committed a crime, the words Kaede said in order to protect everyone were very much real. When Kaede asked everyone to end the Killing Game and be good friends in the outside world, Kirumi said that she would do as she requested before Kaede was killed.

Unfortunately, watching her motive video would cause Kirumi to end up unintentionally betraying Kaede post-mortem after the second motive was presented, being pushed beyond her limit from watching it, killing Ryoma Hoshi and being executed herself during the second Class Trial. When they first met, Shuichi realized who Kirumi really is and was surprised when she mentioned how popular she is because of her talent.

Kirumi seems to care for Shuichi, willing to obey him the same way as the rest of their classmates. She even told Kaito to stop hurting Shuichi after the first trial ended. Kirumi was really disappointed when Shuichi pointed her out as the culprit in the second trial.

Kirumi still hasn't accepted her fate and tried to vote Shuichi as the culprit. Shuichi was depressed and upset when he got to see another friend getting killed. He seems to be also one of those people who tried to tell Kirumi to run away from her execution. In her Free Time Events with Shuichi, Kirumi saw a lot of potential within him and wished to serve him. In the bonus Love Suite scene, Shuichi has a role of being Kirumi's master, with whom she has fallen in love with. At first, she believes these feelings are inappropriate but Shuichi convinces her otherwise.

It's shown in the fantasy that Kirumi still sees Shuichi as the Ultimate Detective being her ideal partner. After Kirumi had discovered through a Kubs Pad given to her in Chapter 2 that she was given a small country to rule over by the Prime Minister of Japan, she made Ryoma her target in order to escape, believing him to be the weakest one among them all. When told to come to his own Research Lab by Kirumi, Ryoma did not hesitate to go along with her plans and willingly turned his back to her, fueling her killing intent.

She hit him over the head, cuffed him, and drowned him. Ryoma seemed to strongly respect Kirumi for her will to live when he himself had none. After learning that she is a great cook, Kokichi wants Kirumi to be his "mom" and often asks her about it. She is not fond of the idea, though. Kokichi is later chased around and scolded by Kirumi. When disappointed in her behavior, Kokichi responds with a "and she dares to call herself my mom" attitude.

When Kirumi is found out to be the culprit, Kokichi does not appear to feel bad for her. When the students question her about lying to them, Kokichi pulls one last mom joke by asking if she isn't his mom after all. After her execution, however, he appears to feel some genuine respect for her, stating that running away in order to live isn't a bad thing and even bawls loudly after witnessing her execution.

In the official art book, it is indicated that Kirumi regularly polished Kokichi's shoes. Kirumi is more considerate towards K1-B0 than most other students are, in fact, she appears to be the most respectful towards him. She considerately asks K1-B0 about his maintenance and in general appears to see him as an equal to humans. When K1-B0 is asked to join Tenko, Himiko and Angie to relax and be pretend celebrities, Kirumi wishes to serve him as well and asks him if he would prefer to have oil.

K1-B0 answers it won't be necessary, and the offer alone made him happy. It's revealed that during the second trial, Keebo noticed what Monosuke said about "last time" that they were asked about standing on the pool's windowsill at night, hinting that the culprit was the one who asked him in order to secretly confirm the details of their murder plan. In which this happens to be true, and that person who asked Monosuke and killed Ryoma was Kirumi Tojo.

Both share the same execution as Monodam shoves Monosuke right under Kirumi's plummeting body, causing both of them to die instantly from the gruesome impact. Danganronpa V3: Killing Harmony English. Free Time Events :. Love Across the Universe :. Ultimate Talent Development Plan :. Danganronpa Wiki Explore. Danganronpa: The Animation Danganronpa 3. Explore Wikis Community Central. Register Don't have an account? Kirumi Tojo. View source. History Talk Do you like this video?

Play Sound. Please let me know if you require my service. I am not amused. As a maid, my only desire is to fulfill the desires of others.

So please, think about what you desire for yourself, and what you desire from me. No matter the situation, no matter what happens, I am here to serve everyone. Until we escape, I intend to serve everyone. If either of you have a request to make of me, please do not hesitate to ask. Even on this uncharted path, we must find our way. Even if she did commit a crime, what Kaede has said to us is not a lie. She wanted to protect us, and I firmly believe that to be the truth.

Chapter 2: "A true maid must be able to adapt to any situation. I, too, intend to fulfill Kaede 's request. So I will valiantly perform any task, trivial or otherwise.

I do not mind. To serve others is a maid's duty. I can cast aside my personal feelings for the sake of serving everyone. Given our situation, I will not take monetary payment. I will do anything you ask. Your happiness is the reason I serve. Serving others is the true desire of any maid.

It is my duty to unveil the truth, so I do not care if people suspect me. Because everyone's lives are at stake. If that is the case, then I will have to deny it. I will not let you make the wrong choice. I will refute your accusations with all my might.. But I cannot allow that! Because I must do this for everyone! I must serve everyone and protect them The OSCC cases included 54 men and 46 women, ranging in age from 39 to 93 years median, Most ppositive cases showed strong and diffuse staining in the nucleus and the cytoplasm of the tumor cells Fig.

The association between p16 immunohistochemistry expression in the tumors from patients with OSCC and their clinicopathological parameters was investigated; however, the differences were not significant Table I. Furthermore, following the analysis between p16 expression and survival using the Kaplan-Meier method, no significant association between overall and disease-free survival times was found Fig.

Immunohistochemical staining for p16 protein in oral squamous cell carcinoma tissues. C A representative image showing negative expression. Association between p16 status and the characteristics of patients with oral squamous cell carcinoma.

The other cases Case 3 and 10 were determined to be keratinized squamous cell carcinomas. Only 1 case was found to be positive. HMBS was used as a loading control.

HPV has been recognized as a possible pathogen of oral cancer 27 — HPV has been associated with cancer of the uterus and cervix and was found in most cases 6 — 8. However, its role in oral carcinogenesis is still unknown 30 — In oropharyngeal HNSCC, HPV-positive tumors may have different clinical and biological functions, with improved overall survival time and favorable prognosis Another study showed that cases positive for HPV had lower recurrence rates compared with that for their negative counterparts, indicating an association between HPV infection and a good prognosis in OSCC Therefore, the association between HPV infection and prognosis could not be evaluated.

Several techniques have been used to detect HPV. HPV detection methods, such as morphology, in situ hybridization and p16 immunohistochemistry lack sensitivity and specificity, as well as the ability to detect high-risk HPV types. Therefore, PCR was considered the most sensitive method 38 , It suggested that although p16 protein expression was a biomarker for cervical or tonsillar cancer arising from carcinogenic HPV infection, it was not applicable for tongue cancer NT and KN confirm the authenticity of all the raw data.

KN designed the experiments. NT and ST performed the experiments. NT and KN wrote the manuscript. All authors read and approved the final version of manuscript.

The present study was approved by the Institute Research Ethics Committee of the Ehime University Hospital approval number, and written informed consent was provided by all the patients. National Center for Biotechnology Information , U. Journal List Oncol Lett v. Oncol Lett. Published online May Author information Article notes Copyright and License information Disclaimer.

Received Nov 27; Accepted Apr This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Keywords: head and neck squamous cell carcinoma, human papillomavirus, infection, oral squamous cell carcinoma, p Results Characteristics of the patients The OSCC cases included 54 men and 46 women, ranging in age from 39 to 93 years median, Open in a separate window. Figure 1. Table I. Figure 2. Discussion HPV has been recognized as a possible pathogen of oral cancer 27 — Supplementary Material Supporting Data: Click here to view.

Authors' contributions NT and KN confirm the authenticity of all the raw data. Ethics approval and consent to participate The present study was approved by the Institute Research Ethics Committee of the Ehime University Hospital approval number, and written informed consent was provided by all the patients.